I'm wondering whether that line up in the trailer shows all of the villains. I've identified everyone I can, feel free to add or comment on the list.
Baby Amelia Airheart
Mama Bosco??? (the one with the afro)
Mole People (Mole builder, occult moles)
Sam Clones? (we're assuming they're clones?)
There's somebody behind the clones and Grandpa Stinky who I can't quite make out...