It's kind of a toss up for me between three scenes, either when Carly dies, when Andy grabs Clem by the hair, or when Lee was saying goodbye to Clem. The first two made me act with my heart. There was no thinking in the responses that came up I was furious and knew what I wanted to do even before the choices came up. When Lee was saying goodbye though, Clem said "Don't leave me" he voice broke just a bit and I lost it. That was probably the hardest part of the game for me to play through. The other parts really showed me strength of emotions I didn't know I could have and least of all about characters in a video game but the last scene with Clem and Lee was a more lasting emotion.
I have some minor issues with episode 5 but the ending sequence was spot on from being reunited with Clem till she leaves you very emotional.